染色质与表观遗传学系列讲座第9场 – Jop Kind Chromatin and Epigenetics biweekly virtual seminar series

发布日期:2021-04-08  浏览次数:852



活动时间 | Time

北京时间2021413日(周二) 20:00-21:00

2021 Apr 13th Tuesday 20:00-21:00 (Beijing Time)

参与方式 | Location

Zoom网络研讨会: 843 6931 3262

Bilibili直播:http://live.bilibili.com/22741871

提示:若想通过问答环节等方式与主讲人交流,请下载并安装国际版zoom客户端,参与zoom网络研讨会。参与研讨会需输入会议码、姓名、邮箱,无需注册或登陆zoom账号。

本场讲座会后不提供公开录像。

Webinar ID: 843 6931 3262

Bilibili Live: http://live.bilibili.com/22741871

To interact with the speaker, please join the meeting via zoom and make sure you have zoom client (international version) installed.

The recording of this webinar will not be provided.

主讲人 | Speaker

Jop Kind

主讲人简介 | Speaker Biography

Jop Kind obtained his PhD in 2008 from the Radboud University in Nijmegen for his research in the lab of Asifa Akhtar at the European Molecular Biology Laboratory (EMBL). Next, Dr. Kind undertook postdoctoral training in the group of Bas van Steensel at the Netherlands Cancer Institute (NKI) to work on 3D chromosome organization. He was one of the first to acknowledge that single-cell information is essential to understand the functioning of complex biological systems. To achieve this, he developed two innovative complementary technologies to visualize and probe 3D positioning of chromosomes in individual cell nuclei. For this work he received the prestigious John Kendrew (EMBL) and Antonie van Leeuwenhoek (NKI) awards. Since his appointment as a research group leader at the Hubrecht institute (2014), he has implemented these and newly developed single-cell methods to study gene-regulation in early mouse embryogenesis. His research team recently made the discovery that following conception spatial positioning of the chromosomes is a key pioneering event in preparation of the embryo for the first activation of gene activities. He was recently awarded an ERC Consolidator grant to continue this line of research.


Jop Kind博士2008年在荷兰的拉德堡德大学获得博士学位,期间在欧洲分子生物学实验室(EMBL)的Asifa Akhtar实验室进行研究。随后,Kind博士在荷兰癌症研究所(NKI)的Bas van Steensel实验室进行了博士后工作,期间致力于对染色体三维组织的研究。他很早就认识到了单细胞信息对于理解复杂生物系统功能的重要性。为了实现研究单细胞信息的目标,他开发了两种互补的新技术(scDamIDm6A-Tracer)来探测并可视化单个细胞核中染色体的3D定位。他因这些工作获得了著名的约翰·肯德鲁(EMBL)和安东尼·范·列文虎克(NKI)奖。自2014年被任命为胡布里支研究所的研究组负责人以来,他致力于应用上述技术及新开发的单细胞方法,对小鼠早期胚胎发育中的基因调控进行研究。他的研究团队最近发现,染色体的空间定位是胚胎基因首次激活准备的关键先驱事件。他于最近获得了ERC欧洲研究委员会(ERC)的资助,以继续进行这一研究。


报告标题 | Title

Measuring combined single-cell epigenetics and transcriptomics with scDam&T

报告摘要 | Abstract

Recent advances in single-cell multi-omics offers new opportunities to derive detailed integrative insights into the molecular functioning of individual cells. We have previously developed scDamID to profile protein-DNA interactions and chromatin accessibility in individual cells and more recently scDam&T to combine such profiles with scRNA-seq in the same cell. Here I will present examples of the implementation of scDam&T to study spatial genome organization in single cells and present a new method to probe histone post-translational modifications (PTMs) and transcriptomics from the same cell.

主讲人发表论文摘选 | Selected Publications

1) Kind J, et al (2015). Genome-wide maps of nuclear lamina interactions in single human cells. Cell 163:134-147.

2) Rooijers K, et al (2019). Simultaneous quantification of protein-DNA contacts and transcriptomes in single cells. Nature Biotechnology 37:766-772.

3) Markodimitraki CM et al (2020). Simultaneous quantification of protein-DNA contacts and transcriptomes in single cells with scDam&T. Nature Protocols 15:1922-1952.

4) Kind J, et al (2013). Single-cell dynamics of genome – nuclear lamina interactions. Cell 153:178-92.

5) Borsos M, et al (2019). Genome-lamina interactions are established de novo in the early mouse embryo. Nature 569:729-733.

往期回顾 | Past Webinars

8场(限时回看将于414日结束)

2021 Mar. 30th   Speaker: Robert G. Roeder

Mechanistic Studies of Transcriptional Regulation in Leukemia and Lymphoma

https://www.bilibili.com/video/BV1XU4y1a7EA/

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