中国细胞生物学学会
2022年全国学术大会·厦门

季维智  JI Weizhi

中国科学院院士，欧洲科学院院士，教授
Member of the Chinese Academy of Sciences(CAS) , Professor

昆明理工大学
Kunming University of Science and Technology

报告题目：灵长类胚胎发育调控与嵌合研究

季维智，男，1950年生，博士生导师，中国科学院院士，欧洲科学院院士。1978-1982年毕业于云南大学生物系；1983-2012年中科院昆明动物研究所研究员，其中1996-2005年任中科院昆明动物研究所第四任所长，2005-2008年任中科院昆明灵长类研究中心主任。现任昆明理工大学特聘教授，灵长类转化医学研究院院长，省部共建非人灵长类生物医学国家重点实验室主任、云南省灵长类生物医学动物重点实验室理事长。

季维智院士长期坚持灵长类生殖发育生物学的研究，围绕早期胚胎发育调控，干细胞多能性和人类疾病的猴模型及致病机理等科学问题，形成了从体外受精、胚胎早期发育、基因编辑以及干细胞等系统研究体系。率先在基因编辑灵长类动物模型取得重大突破，并获得了naïve猴多能性干细胞。实现了灵长类（人和猴）胚胎体外延长培养，解析了灵长类胚胎发育原肠发生与发育的重要事件,首次获得人猴嵌合胚胎。在胚胎发育和干细胞的基础理论、干细胞多能性和疾病研究都有重要意义。曾获“2015年中国细胞生物学学会终身贡献奖”、“2019年何梁何利基金科学与技术进步奖生命科学奖”。季维智院士长期为国家生殖发育、干细胞专家组服务，为中国生殖与干细胞研究作出了突出贡献，也为中国灵长类研究的国际化并跻身于世界先进行列发挥了重要作用。在 Cell， Science, Nature, Cell Stem Cell，PNAS等杂志以通讯作者或第一作者发表 SCI 论文 100 余篇。其中，2014 年在 Cell 发表的基因编辑猴的论文被评价为人类疾病模型建立的里程碑性工作、入选 2014 年世界十大科技进展（MIT Technology Review）、2014 年 Cell 最佳论文(Cell)、 2014 年世界最成功的 8 大事件之一(Nature)。

Member of Chinese Academy of Sciences (CAS)
State Key Laboratory of Primate Biomedical Research
Institute of Primate Translational Medicine
Kunming University of Science and Technology

Prof. JI Weizhi currently is a Member of Chinese Academy of Sciences (CAS), Professor and Director of the State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology. During the last three decades Prof. Ji has been devoted to developing non-human primate disease models and to advance regenerative and developmental biology with the overarching aim to improve human health. His research has focused on the mechanisms of early embryo development regulation, stem cell pluripotency and nonhuman primate models for human neurodevelopmental and neurodegenerative diseases. He has established a world leading primate research platform encompassing cutting edge biotechnology ranging from in vitro fertilization, early embryonic development, gene editing to stem cell lines which allows a unique approach. His lab developed the first gene-modified rhesus and cynomolgus monkeys via Cas9/RNA-mediated gene targeting in 2014, first proved the feasibility to generate chimeric monkeys using ESCs in 2015 and succeeded in extending in vitro culture for monkey in 2019 and for human early embryos in 2020. These gives a rare opportunity to study the developmental events of gastrulation in primates and offers insights into the new discoveries of fundamental theories in embryonic development, stem cells and promotes the development of innovative treatment for neurodevelopmental disorders. Prof. Ji has been providing his expertise in the national committees for reproduction and development biology, stem cells research and has led a series of state key research projects by the Ministry of Science and Technology, China. His research has been published in internationally recognized journals, including Cell, Science, Nature, Cell Stem Cell, PNAS and others, among which the study of the first primates born with genomes engineered by precision gene-targeting methods published in Cell has been regarded as a milestone of animal disease model development and listed as one of the 10 technology breakthrough in 2014 by MIT Technology Review, Best Research Article of 2014 by Cell, one of the 8 Success in science of 2014 by Nature.

近5年发表论文 / Representative publications in the last 5 years：

1. Tao Tan, Jun Wu, Chenyang Si, Shaoxing Dai, Youyue Zhang, Nianqin Sun, E Zhang, Honglian Shao, Wei Si, Pengpeng Yang, Hong Wang, Zhenzhen Chen, Ran Zhu, Yu Kang, Reyna Hernandez-Benitez, Llanos Martinez Martinez, Estrella Nuñez Delicado, W. Travis Berggren, May Schwarz, Zongyong Ai, Tianqing Li, Hongkui Deng, Concepcion Rodriguez Esteban, Weizhi Ji*, Yuyu Niu, Juan Carlos Izpisua Belmonte. Chimeric contribution of human extended pluripotent stem cells to monkey embryos ex vivo. CELL. 184, 2020-2032(2021). 15 April 2021
2. Zongyong Ai, Baohua Niu, Kui Duan, Chenyang Si, Sile Wang, Lifeng Xiang, Xiaoqing Zhu, Qingyuan Zhu, Chun Feng, Yu Yin, Shumei Zhao, Ruize Kong, Weizhi Ji*, Tianqing Li. Modulation of Wnt and Activin/Nodal supports efficient derivation, cloning and suspension expansion of human pluripotent stem cells. BIOMATERIALS. 249:120015. doi: 10.1016/j.biomaterials.2020.120015. Epub. August 2020
3. Fang Wang, Weiqi Zhang, Qiaoyan Yang , Yu Kang , Yanling Fan, Jingkuan Wei , Zunpeng Liu,Shaoxing Dai , Hao Li, Zifan Li, Lizhu Xu , Chu Chu, Jing Qu, Chenyang Si, Weizhi Ji*,Guang-Hui Liu, Chengzu Long, Yuyu Niu. Generation of a Hutchinson–Gilford progeria syndrome monkey model by base editing.
4. Lifeng Xiang, Yu Yin, Yun Zheng, Yanping Ma, Yonggang Li, Zhigang Zhao, Junqiang Guo, Zongyong Ai, Yuyu Niu, Kui Duan, Jingjing He, Shuchao Ren, Dan Wu, Yun Bai, Zhouchun Shang, Xi Dai, Weizhi Ji*, Tianqing Li. A developmental landscape of 3D-cultured human pre-gastrulation embryos. NATURE. 577, 537–542(2020).12 December 2019
5. Yuyu Niu, Nianqin Sun, Chang Li, Ying Lei, Zhihao Huang, Jun Wu, Chenyang Si, Xi Dai, Chuanyu Liu, Longqi Liu, Su Feng, Yu Kang, Wei Si, Hong Wang, E. Zhang, Lu Zhao, Ziwei Li, Xi Luo, Guizhong Cui, Guangdun Peng, Juan Carlos Izpisúa Belmonte, Weizhi Ji*, Tao Tan. Dissecting primate early post-implantation development using long-term in vitro embryo culture. SCIENCE. 366, 6467 (2019). 15 Nov 2019
6. Lei Shi, Xin Luo, Jin Jiang, Yongchang Chen, CirongLiu, Ting Hu, MinLi, QiangLin, Yanjiao Li, Jun Huang, Hong Wang, Yuyu Niu, Yundi Shi, Martin Styner, Jianhong Wang, YiLu, Xuejin Sun, HualinYu, Weizhi Ji*, Bing Su. Transgenic rhesus monkeys carrying the human MCPH1gene copies show human-like neoteny of brain development. NATIONAL SCIENCE REVIEW. 6: 480–493 (2019). 27 March 2019
7. Yiqiang Cui, Yuyu Niu, Jiankui Zhou, Yongchang Chen, Yiwei Cheng, Shangang Li, Zongyong Ai, Chu Chu, Hong Wang, Bo Zheng, Xuejin Chen, Jiahao Sha, Xuejiang Guo, Xingxu Huang, Weizhi Ji*. Generation of a precise Oct4-hrGFP knockin cynomolgusmonkey model via CRISPR/Cas9-assisted homologous recombination. CELL RESEARCH. 28, 383-386 (2018). 12 January 2018
8. Yongchang Chen, Juehua Yu, Yuyu Niu, Dongdong Qin, Hailiang Liu, Gang Li, Yingzhou Hu, Jiaojian Wang, Yi Lu, Yu Kang, Yong Jiang, Kunhua Wu, Siguang Li, Jingkuan Wei, Jing He, Junbang Wang, Xiaojing Liu, Yuping Luo, Chenyang Si, Raoxian Bai, Kunshan Zhang, Jie Liu, Shaoyong Huang, Zhenzhen Chen, Shuang Wang, Xiaoying Chen, Xinhua Bao, Qingping Zhang, Fuxing Li, Rui Geng, Aibin Liang, Dinggang Shen, Tianzi Jiang, Xintian Hu, Yuanye Ma, Weizhi Ji*, Yi Eve Sun. Modeling Rett Syndrome Using TALEN-Edited MECP2 Mutant Cynomolgus Monkeys. CELL. 169(5):945-955.e10 (2017). 18 May 2017
9. Fei Gao, Yuyu Niu, Yi Eve Sun, Hanlin Lu, Yongchang Chen, Siguang Li, Yu Kang, Yuping Luo, Chenyang Si, Juehua Yu, Chang Li, Nianqin Sun, Wei Si, Hong Wang, Weizhi Ji*, Tao Tan. De novo DNA methylation during monkey pre-implantation embryogenesis. CELL RESEARCH. 27(4):526-539 (2017). 24 February 2017.